DOT Drug & Alcohol Testing for Transportation Employers

Stay audit-ready. Avoid costly violations. Protect your operating authority.

We manage DOT-compliant testing programs for safety-sensitive employers, including random consortium management, Clearinghouse compliance, and full audit documentation support.

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DOT-Regulated Employers

Fully managed DOT drug & alcohol testing programs designed to keep safety-sensitive employers compliant with U.S. Department of Transportation regulations across highway, aviation, rail, transit, and pipeline industries. Includes pre-employment, random, post-accident, reasonable suspicion, and return-to-duty requirements.

Non-DOT Employers

When people hear that DOT drug testing is highly regulated, they sometimes assume non-DOT drug testing is less important. For many employers, a well-designed non-DOT drug testing program is one of the most important steps they can take to protect employees, reduce risk, and put safety first.

Individual Testing Services

Confidential lab-based urine, oral fluid, and hair testing for personal or proof of test needs.

Our Services

Delivering accurate, timely, and accessible drug and alcohol testing with integrity.

DOT Drug Testing

Lab-based testing for all needs.

DOT Alcohol Testing

Breath alcohol testing.

Live Scan Fingerprinting

Digital fingerprinting for employment and licensing.

DOT Mobile On-Site Testing

24/7 mobile testing at your workplace or incident site.

DOT Random Testing Program

Complete administration for employer and DOT pools.

FMCSA Clearinghouse

Compliance Support for CDL Drivers.

DOT Drug Testing

Crossroads Drug and Alcohol Testing provides reliable DOT drug testing services for employers, owner-operators, trucking companies, transit agencies, aviation, pipeline, railroad, and other Department of Transportation (DOT) regulated industries. Our certified collection professionals perform FMCSA-compliant drug testing, including pre-employment, random, post-accident, reasonable suspicion, return-to-duty, and follow-up testing. We also offer lab-based drug testing using SAMHSA-certified laboratories for accurate, legally defensible results. Whether you need individual testing or a complete workplace drug testing program, Crossroads helps employers remain compliant with federal DOT regulations while providing fast turnaround times and professional customer service.

DOT Alcohol Testing

Crossroads Drug and Alcohol Testing offers professional DOT alcohol testing using federally approved breath alcohol testing (BAT) equipment performed by qualified Breath Alcohol Technicians (BATs). Our DOT-compliant alcohol testing services support employers with pre-duty, post-accident, reasonable suspicion, return-to-duty, follow-up, and other federally required alcohol testing programs. We help trucking companies, transportation businesses, municipalities, and safety-sensitive employers maintain full compliance with FMCSA, DOT, and federal workplace regulations. With fast scheduling, accurate documentation, and experienced technicians, Crossroads makes DOT alcohol testing simple, reliable, and compliant.

Live Scan Fingerprinting

Crossroads Drug and Alcohol Testing provides fast, secure Live Scan fingerprinting services for employment, licensing, certification, volunteer organizations, and background checks. Our digital fingerprinting system electronically submits fingerprints to the appropriate state and federal agencies, helping reduce processing time while improving accuracy. Whether you need California Live Scan fingerprinting for healthcare, education, childcare, security, real estate, government employment, or professional licensing, our trained staff provides efficient service with minimal wait times. We make the fingerprinting process simple, convenient, and compliant with all applicable agency requirements.

DOT Mobile On-Site Testing

Crossroads Drug and Alcohol Testing offers 24/7 DOT mobile on-site drug and alcohol testing at your workplace, job site, terminal, warehouse, construction project, or incident location. Our experienced mobile collection team travels directly to your business, minimizing employee downtime while ensuring full DOT and FMCSA compliance. Mobile testing is ideal for post-accident testing, reasonable suspicion situations, large hiring events, and remote work locations where immediate testing is critical. We provide professional collection services, rapid response times, and accurate documentation so employers can maintain productivity while meeting all federal workplace testing requirements.

DOT Random Testing Program

Our DOT Random Testing Program provides complete administration for employers required to maintain federally compliant random drug and alcohol testing pools. Crossroads manages employee enrollment, scientifically valid random selections, notifications, testing coordination, reporting, and recordkeeping to ensure your organization remains compliant with FMCSA, FAA, FTA, FRA, PHMSA, and USCG regulations. Whether you manage a small fleet or a large transportation company, our random testing program reduces administrative burden while helping protect your business from costly compliance violations and audits.

FMCSA Clearinghouse

Crossroads Drug and Alcohol Testing provides comprehensive FMCSA Drug & Alcohol Clearinghouse services to help employers and CDL drivers remain compliant with federal regulations. We assist with employer registration, driver queries, annual limited queries, full queries, electronic consent management, reporting requirements, and Clearinghouse compliance support. Our experienced team helps trucking companies understand and meet all FMCSA Clearinghouse requirements while avoiding penalties and ensuring drivers remain eligible to operate commercial motor vehicles. Whether you need ongoing compliance management or assistance with a single registration, Crossroads is your trusted Clearinghouse partner.

About Crossroads

Delivering accurate, timely, and accessible drug and alcohol testing with integrity.

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Our mission is clear: support our clients in protecting their workforce and the public by delivering reliable, compliant drug and alcohol testing programs for employers, employees, and contractors.

Our team has over 25 years of DOT compliance experience. We provide 24/7 on-site and mobile services, DOT and non-DOT program management, and hands-on, owner-level support. As a locally rooted, minority-owned business, we proudly serve transportation companies, government agencies, and individuals with integrity, accuracy, and a compliance-first approach—when and where you need it.

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Quest

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Case Studies

Need Immediate Testing?
We’re Ready to Help.

Whether it’s a same-day requirement, post-accident testing, or a last-minute pre-employment screen, our certified collectors are available to assist you right now.

How Testing Works

Drug and alcohol testing doesn’t need to be confusing. Here’s what to expect when you visit Cross Roads.

1. Choose Your Test Type

Select the test that fits your needs—Individual, Employer, or DOT-compliant testing. Not sure what you need? Our guide will help you decide.

2. Walk In or Send Employees

No appointment required. Visit any of our locations in Los Angeles, Duarte, or West Memphis, or send employees directly for testing.

3. Receive Your Results

Instant results are available for select tests. Laboratory testing is typically completed within 1–3 business days with certified reporting.

What Our Customers are Saying

Byron(owner) was a very polite/patient professional. He was extremely knowledgable about the fingerprinting background check process through the D.O.J. The Crossroads location also does staffing/personal drug testing with results that are presented quickly. The parking was easy to access. Please support our local Foothill businesses!!!
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Alesia Fortunati
A very clean & friendly place for your drug testing needs. I was impressed by the kind and warm demeanor of the staff. If I ever need them again in the future--I will return here. Do not hesitate due to how it looks on Google Maps--it is the complete opposite. Keep up the stellar work!
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Al
After Quest diagnostics wasted hours of my time trying to get a pre-employment screening, I came here and they saw me immediately. I was out in less than 10 minutes. Fast service.
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Andrew Marr
Just met the nicest gentleman here. Please take care sir and thank you for helping waiting on my husband today for his test for work. 😊 very kind! To others, Don't judge the place on the outside, see the kindness inside!
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Carolann

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